Practical sessions in Biochemistry
General concept
At the end of this course, students should be able to perform, interpret normal and abnormal levels of biochemical constituents and correlate with related disease.
Learning objectives
At the end of the course student should be able to
- Describe and identify the importance of following biochemical instruments and methods and uses of them in biochemical lab.
- Phlebectomy & separation of serum
- Preparation of buffers/normal/molar solutions
- Colorimetry.
- Spectrophotometry
- Flamephotometry
- Paper chromatography
- Centrifugation techniques
- Electrophoresis (demonstration only)
- pH-metry
- Blood gas analysis (demonstration only)
- perform qualitative test of
- amino acids & proteins
- carbohydrates
- lipids.
- perform and interpret normal & abnormal levels and correlate with disease conditions of the following quantitative biochemical tests in serum:
- sugar, urea, creatinine, bilirubin cholesterol, uric acid, calcium albumin and total serum protein.
- perform and interpret normal and abnormal levels and correlate with related disease conditions of the following serum enzyme levels in human:- alkaline phosphatase, aspastate aminotransferase, creatine keniase, analyase.
- perform following urine analysis in patients- sugar, albumin, urobilinogen, urobilin and bilirubin pigments.
