Practical sessions in Microbiology

GENERAL CONCEPT

  • Safety precaution: Universal Precautions
  • Lab rules and Regulations
  • Introduction to commonly used lab equipments
  • Microscopy — Uses of different microscopes
  • Care and handling of different microscopes
  • Staining technique
  • Gram stain
  • ZN stain
  • Albert stain
  • Spore and capsule
  • Antibiotic sensitivity testing
  • Hanging drop preparation
  • Sterilization technique
  • Disinfection and Decontamination
  • Culture media, common biochemical tests, Catalase ,Oxidase ,Coagulase and interpretations
  • Demonstrations of GPC, GPB,GNB, GNC, AFB
  • Fungal culture SDA,
  • KOH Preparation, Lactophenol cotton blue, India Ink Preparation
  • Candida, SDA
  • Anaerobic Jar and anaerobic culture

NEURO SENSORY

  • Examination of CSF (Lab diagnosis of Meningitis)
  • Bacteria: N. meningitidis (Different bacteria/viral/fungal/tubercular)
  • India ink preparation (Cryptococcus), tube test.