1. Anatomy
  2. 1. Thoracic cage
    2. Respiratory muscles and Movements
    3. External Nose and Nasal cavity
    4. Paranasal Air Sinuses
    5. Pharynx
    6. Larynx
    7. Trachea
    8. Brochopulmonary segments
    9. Pleura
    10. Lung
    11. Diaphragm
    12. Histology (Epiglottis, Trachea and Lungs)
  3. Biochemistry
  4. 13. Metabolism in the Lungs
    14. Synthesis of Surfactant system in Lungs
    15. Hyaline Membrane Disease (Infant Respiratory Distress Syndrome)
    16. Gaseous Transport and Haemoglobin
    17. Ion Electrolyte Balance
    18. Henderson Hasselbalch Equation
    19. Anion Gap (AG)
    20. Biological Oxidation
    21. Electrolyte Transport Chain
  5. Microbiology
  6. 22. Streptococcus pyogenes
    23. Corynebacterium diphtheria
    24. Streptococcus pneumonia
    25. Haemophilus influenzae
    26. Pseudomonas aeruginosa
    27. Microbacterium tuberculosis
    28. Bordetella pertussis
    29. Whooping cough
    30. Viruses
    31. Orthomyxo viruses
    32. Paramyxoviruses
    33. Respiratory Syncytial Virus (RSV)
    34. Adenovirus
    35. Rhinovirus
    36. Severe Acute Respiratory Syndrome (SARS)
    37. Histoplasma Capsulatum
    38. Aspergillus
    39. Cryptococcus neoformans
    40. Pneumocystis carinii
    41. Parasites
    42. Paragonimus westermani
  7. Pathology
  8. 43. Atelectasis
    44. Tuberculosis
    45. Chronic Obstructive Pulmonary Disease
    46. Emphysema
    47. Bronchial Asthma
    48. Bronchiectasis
    49. Acute Respiratory Distress Syndrome
    50. Hyaline Membrane Disease
    51. Pneumonia
    52. Pneumoconiosis
    53. Bronchogenic Carcinoma
    54. Pleural Lesions
  9. Pharmacology
  10. 55. Antitussives, Expectorants and Mucolytics or Therapy of Cough
    56. Drugs used in Bronchial Asthema
    57. Nasal Decongestants and Cold remedies or Therapy of Allergic Rhinitis
    58. Chemotherapy of Tuberculosis
  11. Physiology
  12. 59. Mechanism and Mechanics of Breathing
    60. Regulation of Respiration
    61. Pulmonary circulation
    62. Pulmonary Gas exchange
    63. Gas transport and Exchange
    64. Lung compliance
    65. Pulmonary Function Tests
    66. Spirometry
    67. Applied Respiratory Physiology
    68. Abnormal Breathing
    69. Artificial Respiration
    70. Lung Defense Mechanism
Microbiology
25. Haemophilus influenzae
INTRODUCTION
HISTORY
VIRULENCE FACTORS
Capsular polysaccharide
Bacterial endotoxin
Outer membrane proteins
IgA1 proteases
Pili
Other adhesion proteins
PATHOGENESIS
CLINICAL MANIFESTATION
LABORATORY DIAGNOSIS
Specimen collection
  1. Sputum
  2. Blood
  3. CSF
  4. Aspirates from joints, middle ears or sinuses
Storage
It is highly sensitive to low temperature, and thus should never be refrigerated.
Transport
It should be transported immediately.
Direct smear microscopy
Gram staining
  1. Gram -ve coccobacilli
  2. Pleomorphic appearance
Capsule detection (Quellung reaction)
Capsular swelling occurs when a drop of CSF is mixed with type b antiserum and methylene blue and observed under microscope.
Antigen detection
The type b capsular antigen can be detected in by
Latex agglutination test
Direct-IF test
ELISA
Culture
Culture medium
    H. influenzae is highly fastidious, requires two accessory growth factors (factor X and V) in blood for their growth.
  1. Factor X is a hemin, present freely in blood
  2. Factor V is nicotinamide adenine dinucleotide (NAD), which is present inside RBCs. It is also produced by some bacteria, such as Staphylococcus aureus.
Basal media
On growth on Nutrient agar and Peptone water as they lack X and V factors.
Enriched media
Blood agar
  1. Growth is scanty is because only factor X is availabe freely in blood agar and factor V is minutely availabe as it is present intracellularly.
Chocolate agar
Grows well on chocolate agar.
Blood agar with S. aureus streak line
Colonies of H. influenzae can grows adjacent to S. aureus streak line- a phenomenon called as Satellitism.
Satellitism
  1. When S. aureus is streaked across a blood agar plate perpendicular to the H. influenzae streak line, factor V is released from S. aureus.
  2. Therefore, it forms larger colonies adjacent to S. aureus streak line and size of the colonies decreases gradually away from the S. aureus streak line.
  3. This phenomenon is called Satellitism, a property that is routinely employed for the isolation of H. influenzae.
Special media
Fildes agar and Levinthal's agar containing factor X and V.
Selective media
Haemophilus selective medium
It contains Bacitracin and Sucrose.
Culture smear microscopy
Gram stain
  1. Gram -ve coccobacilli
  2. Pleomorphic appearance
Hanging drops
Non-motile bacilli
Identification
Biochemical tests
Catase test
+ve
Oxidase test
+ve
Disk test for X and V requirement
Growth around XV disk only
  1. H. influenzae
  2. H. aegyptius
  3. H. haemolyticus
Growth around XV and X disk
  1. H. ducreyi
  2. H. aphrophilus
Growth around XV and V disk
  1. H. parainfluenzae
  2. H. parahaemolyticus
  3. H. paraphrophilus
Automation tests
VITEK
MALDI-TOF
Typing methods
Biotyping
Serotyping
Molecular methods
Multiplex PCR
BioFire Film Array
PROPHYLAXIS
TREATMENT